Amino acid acceptor activity of bacteriophage T4 transfer RNA

Nucleotide sequence determination of bacteriophage T4 leucine transfer ribonucleic acid.

The nucleotide sequence of a T4 tRNA with an anticodon for glycine has been determined using (32)P-labeled material from T4-infected cultures of Escherichiacoli. The sequence is: pGCGGAUAUCGUAUAAUGmGDAUUACCUCAGACUUCCAApsiCUGAUGAUGUGAGTpsiCGAUUCUCAUUAUCCGCUCCA-OH. The 74 nucleotide sequence can be arranged in the classic cloverleaf pattern for tRNAs. The anticodon of T4 tRNA(Gly) is UCC with a p...

Amino-acid acceptor activity of the "70S-associated" 4S RNA from avian myeloblastosis virus.

The "70S-associated" 4S RNA from avian myeloblastosis virus contains transfer RNA, as determined by aminoacylation. The various amino acids esterified, plus the great number of minor bases found in this "70S-associated" 4S RNA indicate that a sizeable fraction, but not all, of this RNA is transfer RNA.

The nucleotide sequence of threonine transfer RNA coded by bacteriophage T4.

The nucleotide sequence of a low molecular weight RNA coded by bacteriophage T4 (and previously identified as species alpha) has been determined. The molecule is of particular biological interest for its associated biosynthetic properties. This RNA is 76 nucleotides in length, contains eight modified bases, and can be arranged in a cloverleaf configuration common to tRNAs. The anticodon sequenc...

Effect of chemical modification of dihydrouridine in yeast transfer ribonucleic acid on amino acid acceptor activity and ribosomal binding.

Dihydrouridine residues in bakers’ yeast transfer RNA were reductively cleaved with sodium borohydride. The amino acid acceptor activity of three species of transfer RNA and the ribosoma binding of one species of transfer RNA were determined for these modified transfer RNAs. The data indicate that the reductive cleavage of the dihydrouridine residues in these transfer RNAs has no effect upon th...

Conservation of Specificity between Amino Acid Acceptor Rna and Amino Acyl-srna Synthetase.